Wound healing occurs by regeneration or repair in which the damaged tissue at the wound site is replaced with fibroblastic mediated scar tissue. However, inadequate cell repair due to poor vascularity at the wound site results in non-healing wounds. This study evaluated the effect of human embryonic stem cell (hESC) therapy in six patients with non-healing wounds. The wound was cleaned and washed with normal saline and cotton gauze. The ready to use form of hESCs was sprayed on the wound and final dressing was done with antibiotics, vaseline gauze and cotton pads. The wounds of all the patients healed after receiving hESC therapy. Reduction in the size of wounds and granulation was observed among all the patients. hESC therapy showed improved and faster wound healing in non-healing wounds of different etiologies.