A new paradigm in allogenic stem cells
A new benchmark:
A cell line with more than 5,000 passages in over 17 years and counting...
ReoStem is a unique, replicable, clinical-stage technology platform for the generation of allogenic stem cells. It covers all stages of cell culture from derivation, expansion, and establishment of cell lines to the preparation of ready-to-use allogenic stem cell products.
The resultant product has genomic stability and universal acceptability.
It has been used to establish two ready-to-use allogenic stem cell products:SCT-N® and SCT-NX®.
ReoStem technology produces cell lines which are capable of differentiating into all the cell types of the body. The suppression of immunogenic genes make universality an important feature of these cell lines. Greater than 90% viability is routinely achieved in the ready-to-use format when kept under temperature-controlled conditions for more than 10 years. There is no contamination with any animal products, bacteria or fungi. Microarray data show that ReoStem cells are genetically stable.
The cell lines were derived from a single, expendable fertilized ovum in the year 2000 from a regular IVF cycle undertaken with due consent. This novel technology was subsequently patented and published.
UNIQUE PROFILE of ReoStem
ReoStem is an allogenic stem cell platform. It combines the following features:
ReoStem platform has derived its cell lines, SCT-N® and SCT-NX®, from a single, expendable fertilized ovum taken from a natural IVF cycle with full and informed consent of the donor.
The cells are cultured in an animal product-free media in suspension. The number of cells greatly increase after incubation.
Expansion and Controlled differentiation
Post incubation, the cells are expanded and selectively primed to create two cell lines, SCT-N® and SCT-NX®, enriched for neuronal and non-neuronal precursors, respectively. Uniquely, ReoStem permits incubation and expansion in a cyclic process.
Preparation of cell lines- SCT-N® and SCT-NX®
These cells can either be stored or re-incubated. The cells are stored at -18°C to -22°C. The cells are centrifuged and re-suspended in clinical grade normal saline. This is the ready to use product.
Storage of SCT-N® and SCT-NX®
The ready to use product should be stored at -18°C to -22°C. Its viability is maintained for more than 10 years.
The end use is for clinical application. The product needs to be thawed before use.